Ameliorative effects of Sargassum stolonifolium amendment on physiological and biochemical parameters in Brassica chinensis L. under cadmium contaminated soil

This research was carried out to examine the effects of Sargassum stolonifolium on reducing cadmium in Brassica chinensis L. tissue, its influential roles physiological parameters and antioxidant mechanism B. exposed stress. Different levels Cd (50 mg 100 mg) with without S. (25g, 50g 100g) under five replications were explored this study. Biomass, photosynthetic pigment, relative water content (RWC), malondialdehyde (MDA), hydrogen peroxide (H2O2), 2,2-diphenyl-1-picrylhydrazyl (DPPH), total activity (TAA), non-protein thiol (NPT), protein (PT), bound thiol, glutathione (GSH), phytochelatins, ascorbate peroxidase (APX), Catalase (CAT), superoxide dismutase (SOD) guaiacol (POD) determined. The results revealed that stress significantly (P < 0.05) reduced plant biomass attributes, accumulated higher concentrations tissues increasing rate concentration soil. However, incorporation at g 50 (T4) spiked soil increased FW (40.6%) DW (72.2%) respective treatment stolonifolium. Similarly, accumulation roots, stem leaves decreased by 90.25%, 82.93% 84.6% respectively compared T1 Cd) thereby leaf MDA H2O2 contents 40.1% 68.8%, respectively, kg−1 T1. An increase noticed chlorophyll a, b, carotenoid, SPAD RWC a value 114.6%, 20.7%, 73.7%, 44.8%, 6.3%, over control (T0). DPPH scavenging TAA 119.8 81.5% percent T0. Concentration increment NPT, TT, GSH PCs 66.7%, 49.1%, 60.1%, 96.1% 3.4% T4 Antioxidant enzymes activities APX (92.8%), CAT (73.1%), SOD (20.9%) POD (88.9%) for control. has potential improve growth defensive system ameliorate phytotoxicity as well immobilization.